Staining of living bacteria with rhodamine 123
نویسندگان
چکیده
منابع مشابه
Localization of mitochondria in living cells with rhodamine 123.
The laser dye rhodamine 123 is shown to be a specific probe for the localization of mitochondria in living cells. By virtue of its selectivity for mitochondria and its fluorescent properties, the detectability of mitochondria stained with rhodamine 123 is significantly improved over that provided by conventional light microscopic techniques. With the use of rhodamine 123, it is possible to dete...
متن کاملFluorescence lifetime imaging (FLIM) of rhodamine 123 in living cells.
A novel setup for fluorescence intensity and lifetime imaging (FLIM) of living cells is reported. Time-resolving techniques are combined with total internal reflection fluorescence microscopy (TIRFM), which permits optical excitation of either plasma membranes or whole cells depending on whether the angle of incidence of the excitation light is greater or smaller than the critical angle for tot...
متن کاملIncreased Rhodamine 123 Uptake by Carcinoma Cells1
The total cellular content of the fluorescent mitochondrialspecific dye rhodamine 123 (Rh-123) was quantified by butanol extraction as a function of time of exposure and dose for a variety of cell lines. These results were compared with observa tions made by fluorescence microscopy on dye localization and mitochondrial morphology. There appeared to be two categories of cell types based on Rh-12...
متن کاملIncreased Rhodamine 123 Uptake by Carcinoma Cells1
The total cellular content of the fluorescent mitochondrialspecific dye rhodamine 123 (Rh-123) was quantified by butanol extraction as a function of time of exposure and dose for a variety of cell lines. These results were compared with observa tions made by fluorescence microscopy on dye localization and mitochondrial morphology. There appeared to be two categories of cell types based on Rh-12...
متن کاملConjugates of a Photoactivated Rhodamine with Biopolymers for Cell Staining
Conjugates of the photoactivated rhodamine dyes with biopolymers (proteins, polysaccharides, and nucleic acids) are important tools for microscopic investigation of biological tissue. In this study, a precursor of the photoactivated fluorescent dye (PFD) has been successfully used for staining of numerous mammalian cells lines and for conjugate formation with chitosan ("Chitosan-PFD") and histo...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1984
ISSN: 0378-1097,1574-6968
DOI: 10.1111/j.1574-6968.1984.tb00202.x